Resposta da cultura do feijoeiro à adubação nitrogenada e à inoculação com rizóbio.

bitstream/item/67364/1/34249.pdfOrganizado por: Fábio Martins Mercante e Oscar Fontão de Lima Filho. RELARE

Delineating limits: Confronting predicted climatic suitability to field performance in mistletoe populations

1. Biotic stressors such as hemiparasites have a profound impact on forest functioning. However, predicting the future incidence of these stressors on forests remains challenging because climate-based distribution does not consider tree-hemiparasite interactions or the impacts of extreme climate events on stressors'' performance. 2. We use species distribution models (SDMs) and ecophysiological and demographic field data to assess whether climatic suitability is a proxy for the performance of the hemiparasite mistletoe (Viscum album) in two forests with contrasting climatic conditions. Two host tree species representing wet-cold (Scots pine) and dry-warm (Aleppo pine) conditions were selected. We fitted SDMs based on climate variables, and measured different ecophysiological variables capturing cold- (photoinhibition) and drought tolerance (intrinsic water-use efficiency, iWUE). We also assessed demographic variables related to seed germination and establishment rates of mistletoe through a translocation experiment. 3. Species distribution models showed a high climatic suitability of mistletoe in both forests. Mistletoes living in the Scots pines site presented a higher cold tolerance, while those inhabiting the Aleppo pine site showed a higher iWUE. Seedlings coming from local seeds showed a lower mortality than seedlings coming from translocated seeds. Germination and seedling establishment showed temporal mismatches when comparing local and translocated seeds. 4. Synthesis. Habitat suitability predicted by SDMs based on climate data and field performance were related in this mistletoe species. However, ecophysiological and demographic variables indicated a lower fitness of mistletoe in the dry-warm site associated with drought stress. In conclusion, predicted climate suitability based on SDMs forecasts should be refined using field data on actual performance and considering plant-to-plant interactions and extreme climate events

Discordant bioinformatic predictions of antimicrobial resistance from whole-genome sequencing data of bacterial isolates: an inter-laboratory study.

Antimicrobial resistance (AMR) poses a threat to public health. Clinical microbiology laboratories typically rely on culturing bacteria for antimicrobial-susceptibility testing (AST). As the implementation costs and technical barriers fall, whole-genome sequencing (WGS) has emerged as a 'one-stop' test for epidemiological and predictive AST results. Few published comparisons exist for the myriad analytical pipelines used for predicting AMR. To address this, we performed an inter-laboratory study providing sets of participating researchers with identical short-read WGS data from clinical isolates, allowing us to assess the reproducibility of the bioinformatic prediction of AMR between participants, and identify problem cases and factors that lead to discordant results. We produced ten WGS datasets of varying quality from cultured carbapenem-resistant organisms obtained from clinical samples sequenced on either an Illumina NextSeq or HiSeq instrument. Nine participating teams ('participants') were provided these sequence data without any other contextual information. Each participant used their choice of pipeline to determine the species, the presence of resistance-associated genes, and to predict susceptibility or resistance to amikacin, gentamicin, ciprofloxacin and cefotaxime. We found participants predicted different numbers of AMR-associated genes and different gene variants from the same clinical samples. The quality of the sequence data, choice of bioinformatic pipeline and interpretation of the results all contributed to discordance between participants. Although much of the inaccurate gene variant annotation did not affect genotypic resistance predictions, we observed low specificity when compared to phenotypic AST results, but this improved in samples with higher read depths. Had the results been used to predict AST and guide treatment, a different antibiotic would have been recommended for each isolate by at least one participant. These challenges, at the final analytical stage of using WGS to predict AMR, suggest the need for refinements when using this technology in clinical settings. Comprehensive public resistance sequence databases, full recommendations on sequence data quality and standardization in the comparisons between genotype and resistance phenotypes will all play a fundamental role in the successful implementation of AST prediction using WGS in clinical microbiology laboratories

Whole Genome Multi-Locus Sequence Typing and Genomic Single Nucleotide Polymorphism Analysis for Epidemiological Typing of Pseudomonas aeruginosa From Indonesian Intensive Care Units

We have previously studied carbapenem non-susceptible Pseudomonas aeruginosa (CNPA) strains from intensive care units (ICUs) in a referral hospital in Jakarta, Indonesia (Pelegrin et al., 2019). We documented that CNPA transmissions and acquisitions among patients were variable over time and that these were not significantly reduced by a set of infection control measures. Three high risk international CNPA clones (sequence type (ST)235, ST823, ST357) dominated, and carbapenem resistance was due to carbapenemase-encoding genes and mutations in the porin OprD. Pelegrin et al. (2019) reported core genome analysis of these strains. We present a more refined and detailed whole genome-based analysis of major clones represented in the same dataset. As per our knowledge, this is the first study reporting Single Nucleotide Polymorphisms (wgSNP) analysis of Pseudomonas strains. With whole genome-based Multi Locus Sequence Typing (wgMLST) of the 3 CNPA clones (ST235, ST357 and ST823), three to eleven subgroups with up to 200 allelic variants were observed for each of the CNPA clones. Furthermore, we analyzed these CNPA clone clusters for the presence of wgSNP to redefine CNPA transmission events during hospitalization. A maximum number 35350 SNPs (including non-informative wgSNPs) and 398 SNPs (ST-specific_informative-wgSNPs) were found in ST235, 34,570 SNPs (including non-informative wgSNPs) and 111 SNPs (ST-specific_informative-wgSNPs) in ST357 and 26,443 SNPs (including non-informative SNPs) and 61 SNPs (ST-specific_informative-wgSNPs) in ST823. ST-specific_Informative-wgSNPs were commonly noticed in sensor-response regulator genes. However, the majority of non-informative wgSNPs was found in conserved hypothetical proteins or in uncharacterized proteins. Of note, antibiotic resistance and virulence genes segregated according to the wgSNP analyses. A total of 8 transmission chains for ST235 strains followed by 9 and 4 possible transmission chains for ST357 and ST823 were traceable on the basis of pairwise distances of informative-wgSNPs (0 to 4 SNPs) among the strains. The present study demonstrates the value of detailed whole genome sequence analysis for highly refined epidemiological analysis of P. aeruginosa

Cenários de desenvolvimento sustentável no Pantanal em função de tendências hidroclimáticas.

Nesta feita uma análise prospectiva do futuro hidrológico do Pantanal sob a perspectiva de dados pretéritos, da mudança do clima com base em relações entre o nível máximo do Rio Paraguai, interação oceano-atmosfera e precipitação na América do Sul. A cenarização hidroclimática, incluindo a componente humana, permite ainda identificar adaptações e oportunidades para as próximas décadas, também em função de mudanças e tendências da economia mundial.bitstream/CPAP-2009-09/56873/1/DOC98.pd

Effect of Maternal Dietary Condensed Tannins from Sainfoin (Onobrychis viciifolia) on Gut Health and Antioxidant-Immune Crosstalk in Suckling Lambs

Ewes fed sainfoin (a source of condensed tannins "CT") may influence the homeostasis of the gastrointestinal tract of suckling lambs. This study investigated the effects of CT from sainfoin in the maternal diet on plasma fructosamine, faecal coccidian excretion, and gene expression of immune and antioxidant markers in jejunum and ileum of suckling lambs. Twelve Rasa Aragonesa lambs with their dams were selected. The maternal diet was based on fresh sainfoin (SAINFOIN, n = 6) and sainfoin + polyethylene-glycol (SAINFOIN + PEG, as a CT-binder, n = 6) plus a daily supplement of 200 g barley in both groups. A lower percentage of lambs that shed more than 10 oocysts/g faeces was observed in SAINFOIN compared to the SAINFOIN + PEG group (p = 0.07). Jejunal gene expression of transforming growth factor-β1, tumour necrosis factor-α, and glutathione peroxidase (GPX) 1 and 4 were lower in the SAINFOIN group (p < 0.05). In contrast, ileal catalase and GPX2 expression were increased in the SAINFOIN group (p < 0.05). Overall, the results suggest that the presence of CT in the dams' diets has a positive effect on reducing excreted coccidian oocysts and favours antioxidant-immune crosstalk at gut level in suckling lambs.This research was funded by the Ministry of Science, Innovation and Universities of Spain and the European Regional Development Funds, grant number INIA RTA2017-00008-C02-01 and 02

Deciduous and evergreen oaks show contrasting adaptive responses in leaf mass per area across environments

Increases in leaf mass per area (LMA) are commonly observed in response to environmental stresses and are achieved through increases in leaf thickness and/or leaf density. Here, we investigated how the two underlying components of LMA differ in relation to species native climates and phylogeny, across deciduous and evergreen species. Using a phylogenetic approach, we quantified anatomical, compositional and climatic variables from 40 deciduous and 45 evergreen Quercus species from across the Northern Hemisphere growing in a common garden. Deciduous species from shorter growing seasons tended to have leaves with lower LMA and leaf thickness than those from longer growing seasons, while the opposite pattern was found for evergreens. For both habits, LMA and thickness increased in arid environments. However, this shift was associated with increased leaf density in evergreens but reduced density in deciduous species. Deciduous and evergreen oaks showed fundamental leaf morphological differences that revealed a diverse adaptive response. While LMA in deciduous species may have diversified in tight coordination with thickness mainly modulated by aridity, diversification of LMA within evergreens appears to be dependent on the infrageneric group, with diversification in leaf thickness modulated by both aridity and cold, while diversification in leaf density is only modulated by aridity

The Second Transmembrane Domain of P2X7 Contributes to Dilated Pore Formation

Activation of the purinergic receptor P2X7 leads to the cellular permeability of low molecular weight cations. To determine which domains of P2X7 are necessary for this permeability, we exchanged either the C-terminus or portions of the second transmembrane domain (TM2) with those in P2X1 or P2X4. Replacement of the C-terminus of P2X7 with either P2X1 or P2X4 prevented surface expression of the chimeric receptor. Similarly, chimeric P2X7 containing TM2 from P2X1 or P2X4 had reduced surface expression and no permeability to cationic dyes. Exchanging the N-terminal 10 residues or C-terminal 14 residues of the P2X7 TM2 with the corresponding region of P2X1 TM2 partially restored surface expression and limited pore permeability. To further probe TM2 structure, we replaced single residues in P2X7 TM2 with those in P2X1 or P2X4. We identified multiple substitutions that drastically changed pore permeability without altering surface expression. Three substitutions (Q332P, Y336T, and Y343L) individually reduced pore formation as indicated by decreased dye uptake and also reduced membrane blebbing in response to ATP exposure. Three others substitutions, V335T, S342G, and S342A each enhanced dye uptake, membrane blebbing and cell death. Our results demonstrate a critical role for the TM2 domain of P2X7 in receptor function, and provide a structural basis for differences between purinergic receptors. © 2013 Sun et al